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Research

Confocal laser scanning microscope
Confocal laser scanning microscope
Scrape loading technique
Scrape loading technique
Patch clamp technique
Patch clamp technique

Topics:

The group of cell physiology and cellular mechanics under the supervion of Anaclet Ngezahayo work on the following topics

  • Gap Junction dependent cell-to-cell communication
  • Purinergic receptors
  • Ion channels
  • Tissue Engineering

Following cell systems are in the focus of our research:

  • The microvascukar system of the CNS (Blood-Brain-Barriere)
  • The cardiovascular system
  • The glia cells of the CNS
  • The bone forming cells

Techniques:

Electrophysiological methods / imaging-techniques

  • Patch clamp technique
  • Two electrode voltage clamp technique
  • Scrape-loading /Dye transfer technique
  • Calcium-Imaging
  • Confocal Laser Scanning Microscopy

Biochemical methods

  • Protein isolation
  • Western Blot
  • Comet Assay
  • BrdU / MTT Assay

Molecular biological methods

  • cloning / site directed mutagenesis
  • mRNA-synthesis
  • RNAi
  • Semiquantitative RT-PCR
  • Transfection

 

S. Publications